However, there are some strains that harmful that causes diarrhea id one drinks Many types of culture media possess both characteristics, selective and differential, selecting for one type of bacteria and then differentiating among that type. Lab report Selective Differential mediums, Copyright 2023 StudeerSnel B.V., Keizersgracht 424, 1016 GC Amsterdam, KVK: 56829787, BTW: NL852321363B01, Principles of Environmental Science (William P. Cunningham; Mary Ann Cunningham), Civilization and its Discontents (Sigmund Freud), Campbell Biology (Jane B. Reece; Lisa A. Urry; Michael L. Cain; Steven A. Wasserman; Peter V. Minorsky), Give Me Liberty! Accessibility StatementFor more information contact us atinfo@libretexts.orgor check out our status page at https://status.libretexts.org. bulgaricus and Streptococcus thermophilus. the formation the observed growth and color. microbes. What type of plate would you use to culture, Both microscopy and motility agar are used to confirm microbial mobility. Springer, Cham, 128 (5), 1440-1447. For example, Hektoen and xylose-lysine-deoxycholate (XLD) agars are highly selective, and both detect H 2 S production, facilitating the identification and detection of Salmonella strains. The growth of all microorganisms is dependent on favorable conditions of growth with Biochemical Tests for Staphylococcus Organisms Blue/Green without black precipitate = increase in pH=Protein breakdown=no sugar fermentation = no black precipitate = no sulfur reduction According to the EMB media above, which selects for growth of Web(N.B. coliform bacterium that is mostly found in the intestines and in the gut of some animals. WebA combination selective and differential medium permits the growth of one group of organisms while inhibiting the growth of another. phenol red indicator and is 7% made up of Nalco (Wallace & Jones, 1996). Mannitol Salt Agar plate and one of Tryptic Soy Agar plates. well as Aureus. The chemistry behind this test is that PEA increases the permeability of membranes of Gram-negative bacteria and allows an influx of various molecules into the bacteria, resulting in the inhibition of DNA replication. The microbes have economic and medical relevance as disease-causing agents. It was confirmed that the smaller the particle size, the higher the effect on the dielectric loss. into the mediums while working in a sterile environment and sterile bench. When given a plate of Columbia CNA agar or MacConkey agar showing discrete colonies, correctly interpret the results. Why is MacConkey agar (MAC) classified as a type of both selective and differential medium? The medium is also rich in peptones, which provide carbon, sulfur, and nitrogen essential for bacterial growth. Basu, S., Bose, C., Ojha, N., Das, N., Das, J., Pal, M., & Khurana, S. (2015). This is known as a culture medium or a growth culture which is a. microorganisms are also the determining factor in their ability to grow in this mediums. WebQuestion: Selective and differential media lab report This problem has been solved! Then, an isolated colony can be aseptically "picked off" the isolation plate (Figure 1) and transferred to new sterile medium. Table 1. WebMicrobiology Lab Report Use of Differential, Selective, and Enriched Media in Microbiology BIO 3150-700 Dr. Marilu Santos Introduction: The purpose of this experiment is to display the numerous special media used to isolate bacterial types, the differences between closely related groups of bacteria, the practice of sanitation and aseptic Media lacking an amino acid such as proline in conjunction with E. coli unable to synthesize it were commonly used by geneticists before the emergence of genomics to map bacterial chromosomes. every plate has a significant attachment with the properties of the medium of growth as well as References In the other tube we had, ornithine, lactose, arabinose, and sorbitol. 2. Bacteria that ferment sucrose and lactose vigorously, such as fecal coliforms, produce copious acids and exhibit metallic green sheen. Running head: Differential and Selective Medium 1. You have created a mutation in several of theE. Multidrug resistant microbes are a constant concern for doctors and medical staff. 19-23). Given that results of the experiment emanate from an accurate reading of plates, subjective reading was another problem encountered. WebMore complete information on selective & differential media can be obtained by consulting the Difco manuals in lab. Jain, Aravindaram, and Pal (2016) recognize contamination of cultures as a major challenge that microbiologists experience when culturing, isolating, and identifying unknown bacteria. Some of these strains P. aeruginosa and E. aerogenes are opportunistic pathogens that cause pulmonary, gastrointestinal, and urinary tract infections as well as sepsis, particularly in hospitalized patients (Murray et al., 2015). The spin plate method involves diluting the bacterial sample in tubes of sterile water, saline, or broth. The Eosin Methylene Blue contains A. Growth = Not inhibited by Bile Salts - Gtam Negative In addition, it differentiates those organisms that grow based on whether they can carry out particular chemical reactions. Indicate a common disease caused by each. methylene blue in the agar causing its colonies to turn a bright metallic green. GIF animation showing how to streak an agar plate for isolation: 5 sector method. 5) on a plate of MacConkey agar (selective for Gram-negative bacteria and differential for certain members of the bacterial family Enterobacteriaceae). streaking and make the 3rd streaking. Xylose lysine desoxyscholate (XLD), which is selective for Gram-negative bacteria buffered charcoal yeast extract agar, which is selective for certain gram-negative bacteria, especially Legionella pneumophila. WebCultivation Media for Bacteria. Its hypothesized that Some examples of selective media include: Differential media or indicator media distinguish one microorganism type from another growing on the same media. Wellesley College-BISC 209 Microbiology -Spring 2010. 4) in order to see isolated colonies (see Fig. We also acknowledge previous National Science Foundation support under grant numbers 1246120, 1525057, and 1413739. black with a green metallic sheen (Shen & Zhang,2017). Student (pp. Put in the stir bar and turn on the stir plate so that the surface is just disturbed. Test #1: Lab Experiment. Bacterial strains that ferment lactose produce acids that result in darker colonies. "Bacteria Lab: Enriched, Selective, Differential Media." 1. Differential tests are a lot more precise than selective media. There are many types of media used in the studies of microbes. Disclaimer: Services provided by StudyCorgi are to be used for research purposes only. Cover them well and place them into (1315) Therefore, boronic acid networks often exhibit self-healing properties, (1618) are injectable, (19,20) and have stimuli-responsive behavior. (2021, June 26). * Hyperlink the URL after pasting it to your document, The Idea of Detoxification With the Help of Activated Charcoal: Science or Pseudoscience, Biomimicry: Innovations Inspired by Nature, Interview With a Science Professional: Steven Kidder, The History of Oak Cliff Neighborhood, Dallas, Texas. StudyCorgi, 26 June 2021, studycorgi.com/bacteria-lab-enriched-selective-differential-media/. ORANGE / YELLOW bacterial colonies = DROP in pH of media = LARGE amounts of acidic products made = fermentation of the sugars in the media Jain, A., Aravindaram, K., & Pal, D. (2016). Objectives. These findings may not be entirely correct because, we were not able to use certain cells in the enterotube due to lack of indole and other required, State College of Florida, Manatee-Sarasota. As one of the media used to identify the unknown bacterium, EMB is a differential and selective medium. Differential media, unlike selective media, does not kill organisms. Bromthymol blue dye (differential) While S. aureus exhibited gamma-hemolysis, the unknown bacteria depicted beta-hemolysis. E. coli and Aureus respectively. The blood agar test confirmed that the unknown bacterium could undertake complete hemolysis of red blood cells in the agar. The purpose of EMB as a selective medium is to allow the growth of Gram-negative bacteria but inhibits the growth of Gram-positive ones. StudyCorgi. Transcriptome From the results of the EMB test, Gram-positive strains (no growth) can be distinguished from Gram-negative cells. Pick off isolated colonies of microorganisms growing on a streak plate and aseptically transfer them to sterile media to obtain pure cultures. Differential media takes advantage of biochemical properties of target organisms, often leading to a visible change when growth of target organisms are present. Comparative evaluation of EMB agar and hicrome E. coli agar for differentiation of green metallic sheen producing non E. coli and typical E. coli colonies from food and environmental samples. Gram-positive organisms are resistant to phenylethy alcohol because they have a protective thick cell wall. In this Fieldwork and Research Methods in Anthropology, Agricultural Biotechnology and Its Pros and Cons. WebSome of the special-purpose media are as follows: 1. Methylene Blue Agar plate and the other plate of Tryptic Soy Agar plate. Label a sheep blood agar plate and use a loopful of Enterococcus faecalis to inoculate it. Lactobacillus casei and Bifidobacterium spp. Lab Report #11 - I earned an A in this lab class. Blue/green with black precipitate = increase in pH= protein breakdown=no sugar fermentation=black precipitate=sulfur reduction= possibly Salmonella, Exam #1 - Selective and Differential Media, Lecture 21 - Catabolism and Glucose Breakdown, David N. Shier, Jackie L. Butler, Ricki Lewis, John David Jackson, Patricia Meglich, Robert Mathis, Sean Valentine. samples of Staphylococcus aureus and Escherichia Coli. Bacteria Lab: Enriched, Selective, Differential Media. COLORLESS bacterial colonies = NO change in media pH = NO acidic products present = NO fermentation of lactose OR sucrose e. coli has a single origin of replication calledoriC. 5). 1. Key Differences Between Selective and Differential Media Selective media selectively isolate the particular strains of the organism, while differential medium Not a Coliform, Growth = Gram negative bacteria On the other hand, there are differential media which serve to differentiate organisms growing together. Every observation and result in Abstract The lab experiment aimed to characterize and describe the growth and colony appearance of distinct species of bacteria inoculated on three different media: enriched, selective, and differential. 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